{"product_id":"elisa-kit-for-pdz-and-lim-domain-protein-1-pdlim1-sem001hu","title":"ELISA Kit for PDZ And LIM Domain Protein 1 (PDLIM1) - SEM001Hu","description":"ELISA Kit for PDZ And LIM Domain Protein 1 (PDLIM1)\n\nSizes: 24T, 48T, 96T\n\nCatalogue Numbers: SEM001Hu-24, SEM001Hu-48, SEM001Hu-96\n\nCitations, Manuals and MSDS Available upon request.\n\nFeatured Series: Sandwich kit\n\nTarget: PDZ And LIM Domain Protein 1\n\nAlternative Names: CLIM1; CLP-36; CLP36; ELFIN; hCLIM1; Carboxyl Terminal LIM Domain Protein 1; C-terminal LIM domain protein 1; LIM domain protein CLP-36\n\nTarget Species: Human\n\nUniprot: O00151\n\nGene ID: 9124\n\nFeatured Series Function: Detects protein (regular version)\n\nSpecificity: Reactive with Human PDLIM1 \/ PDZ And LIM Domain Protein 1\n\nMethod: Colormetric\n\nDetection principle: Double-antibody Sandwich\n\nDetection\nrange: 0.156-10ng\/mL\n\nSensitivity: 0.061ng\/mL\n\nAssay Time: 3h\n\nSample Size: 100uL\n\nRecommended\/Predicted\nSample Types: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids\n\nAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\n\nStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\n\nShelf-life: 12 months\n\nSpecificity: This assay has high sensitivity and excellent specificity for detection of PDZ And LIM Domain Protein 1 (PDLIM1).\nNo significant cross-reactivity or interference between PDZ And LIM Domain Protein 1 (PDLIM1) and analogues was observed.\n\nPrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PDZ And LIM Domain Protein 1 (PDLIM1) were tested 20 times on one plate, respectively.\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level PDZ And LIM Domain Protein 1 (PDLIM1) were tested on 3 different plates, 8 replicates in each plate.\nCV(%) = SD\/meanX100\n\nStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\n\nTarget: 1. Prepare all reagents, samples and standards;\n2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;\n3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;\n4. Aspirate and wash 3 times;\n5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\n6. Aspirate and wash 5 times;\n7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\n8. Add 50µL Stop Solution. Read at 450nm immediately.\n\nTest principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDZ And LIM Domain Protein 1 (PDLIM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PDZ And LIM Domain Protein 1 (PDLIM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDZ And LIM Domain Protein 1 (PDLIM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDZ And LIM Domain Protein 1 (PDLIM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\n\nResearch Area: Signal transduction; \n\nResearch Use Only","brand":"Cloud-Clone","offers":[{"title":"24T","offer_id":45383787348147,"sku":"SEM001Hu-24","price":431.2,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":45383787380915,"sku":"SEM001Hu-48","price":754.6,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45383787413683,"sku":"SEM001Hu-96","price":1078.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/elisa-kit-for-pdz-and-lim-domain-protein-1-pdlim1-sem001hu","provider":"AFSBio Inc.","version":"1.0","type":"link"}