{"product_id":"elisa-kit-for-tachykinin-precursor-1-tac1-cec780hu","title":"ELISA Kit for Tachykinin, Precursor 1 (TAC1) - CEC780Hu","description":"ELISA Kit for Tachykinin, Precursor 1 (TAC1)\n\nSizes: 24T, 48T, 96T\n\nCatalogue Numbers: CEC780Hu-24, CEC780Hu-48, CEC780Hu-96\n\nCitations, Manuals and MSDS Available upon request.\n\nFeatured Series: Competitive kit\n\nTarget: Tachykinin, Precursor 1\n\nAlternative Names: NK2; NKNA; NKA; TAC2; •PPT; Protachykinin-1; Substance P; Neurokinin A; Neuromedin L; Substance K; Neuropeptide K; Neuropeptide gamma; C-terminal-flanking peptide\n\nTarget Species: Human\n\nUniprot: P20366\n\nGene ID: 6863\n\nFeatured Series Function: Detects small molecule\n\nSpecificity: Reactive with Human TAC1 \/ Tachykinin, Precursor 1\n\nMethod: Colormetric\n\nDetection principle: Competitive Inhibition\n\nDetection\nrange: 123.5-10,000pg\/mL\n\nSensitivity: 51.9pg\/mL\n\nAssay Time: 2h\n\nSample Size: 50uL\n\nRecommended\/Predicted\nSample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids\n\nAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\n\nStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\n\nShelf-life: 12 months\n\nSpecificity: This assay has high sensitivity and excellent specificity for detection of Tachykinin, Precursor 1 (TAC1).\nNo significant cross-reactivity or interference between Tachykinin, Precursor 1 (TAC1) and analogues was observed.\n\nPrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tachykinin, Precursor 1 (TAC1) were tested 20 times on one plate, respectively.\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tachykinin, Precursor 1 (TAC1) were tested on 3 different plates, 8 replicates in each plate.\nCV(%) = SD\/meanX100\n\nStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\n\nTarget: 1. Prepare all reagents, samples and standards;\n2. Add 50µL standard or sample to each well.\n And then add 50µL prepared Detection Reagent A immediately.\n Shake and mix. Incubate 1 hour at 37°C;\n3. Aspirate and wash 3 times;\n4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\n5. Aspirate and wash 5 times;\n6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\n7. Add 50µL Stop Solution. Read at 450 nm immediately.\n\nTest principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tachykinin, Precursor 1 (TAC1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tachykinin, Precursor 1 (TAC1) and unlabeled Tachykinin, Precursor 1 (TAC1) (Standards or samples) with the pre-coated antibody specific to Tachykinin, Precursor 1 (TAC1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tachykinin, Precursor 1 (TAC1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tachykinin, Precursor 1 (TAC1) in the sample.\n\nResearch Area: Neuro science; \n\nResearch Use Only","brand":"Cloud-Clone","offers":[{"title":"24T","offer_id":45379731947699,"sku":"CEC780Hu-24","price":392.0,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":45379731980467,"sku":"CEC780Hu-48","price":686.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45379732013235,"sku":"CEC780Hu-96","price":980.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/elisa-kit-for-tachykinin-precursor-1-tac1-cec780hu","provider":"AFSBio Inc.","version":"1.0","type":"link"}