{"product_id":"elisa-kit-for-trimethylamine-oxide-tmao-cex310ge","title":"ELISA Kit for Trimethylamine Oxide (TMAO) - CEX310Ge","description":"\u003cp\u003eELISA Kit for Trimethylamine Oxide (TMAO)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: CEX310Ge-24, CEX310Ge-48, CEX310Ge-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series: Competitive kit\u003c\/p\u003e\n\n\u003cp\u003eTarget: Trimethylamine Oxide\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: General species\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series Function: Detects small molecule\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Reactive with General species TMAO \/ Trimethylamine Oxide\u003c\/p\u003e\n\n\u003cp\u003eMethod: Colormetric\u003c\/p\u003e\n\n\u003cp\u003eDetection principle: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eDetection\u003cbr\u003e\nrange: 160-100,000pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 61pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 2h\u003c\/p\u003e\n\n\u003cp\u003eSample Size: 50uL\u003c\/p\u003e\n\n\u003cp\u003eRecommended\/Predicted\u003cbr\u003e\nSample Types: Biological Fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\n\u003cp\u003eStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\n\n\u003cp\u003eShelf-life: 12 months\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of Trimethylamine Oxide (TMAO).\u003cbr\u003e\nNo significant cross-reactivity or interference between Trimethylamine Oxide (TMAO) and analogues was observed.\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Trimethylamine Oxide (TMAO) were tested 20 times on one plate, respectively.\u003cbr\u003e\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Trimethylamine Oxide (TMAO) were tested on 3 different plates, 8 replicates in each plate.\u003cbr\u003e\nCV(%) = SD\/meanX100\u003c\/p\u003e\n\n\u003cp\u003eStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cbr\u003e\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\n\n\u003cp\u003eTarget: 1. Prepare all reagents, samples and standards;\u003cbr\u003e\n2. Add 50µL standard or sample to each well.\u003cbr\u003e\n And then add 50µL prepared Detection Reagent A immediately.\u003cbr\u003e\n Shake and mix. Incubate 1 hour at 37°C;\u003cbr\u003e\n3. Aspirate and wash 3 times;\u003cbr\u003e\n4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\u003cbr\u003e\n5. Aspirate and wash 5 times;\u003cbr\u003e\n6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\u003cbr\u003e\n7. Add 50µL Stop Solution. Read at 450 nm immediately.\u003c\/p\u003e\n\n\u003cp\u003eTest principle: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to TMAO has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled TMAO and unlabeled TMAO (Standards or samples) with the pre-coated antibody specific to TMAO. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of TMAO in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of TMAO in the sample.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Cloud-Clone","offers":[{"title":"24T","offer_id":45379594977459,"sku":"CEX310Ge-24","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":45379595010227,"sku":"CEX310Ge-48","price":784.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45379595042995,"sku":"CEX310Ge-96","price":1120.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/elisa-kit-for-trimethylamine-oxide-tmao-cex310ge","provider":"AFSBio Inc.","version":"1.0","type":"link"}