{"product_id":"en2-polyclonal-antibody-bs77280","title":"EN2 Polyclonal Antibody-BS77280","description":"EN2 Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers:  BS77280-50, BS77280-100\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSwiss-Prot: P19622\n\nHost: Rabbit\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB\n\nAll Applications: WB,1:500 - 1:2000\n\nBackground: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Unmodification \n\nExtra Notes: Western blot analysis of extracts of various cell lines, using EN2 antibody at 1:1000 dilution.\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\nLysates\/proteins: 25ug per lane.\nBlocking buffer: 3% nonfat dry milk in TBST.\nDetection: ECL Basic Kit.\nExposure time: 90s.\n\nNote: For research use only, not for use in diagnostic procedure.\n\nBioworld Molecular Weight: 33kDa\n\nAlternative Name: EN2\n\nImmunogen: A synthetic peptide of human EN2(NP_001418.2).\n\nConjugate: Unconjugated\n\nModification: Unmodified ","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42924615073971,"sku":"BS77280-50","price":334.88,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42924615106739,"sku":"BS77280-100","price":576.38,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_77556d85-d100-4ded-a8f4-9235ca56cd60.jpg?v=1703937005","url":"https:\/\/afsbio.com\/products\/en2-polyclonal-antibody-bs77280","provider":"AFSBio Inc.","version":"1.0","type":"link"}