{"product_id":"ep4-polyclonal-antibody-bs66155","title":"EP4 Polyclonal Antibody-BS66155","description":"\u003cp\u003eEP4 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS66155-50, BS66155-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P35408\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB\t 1:500 - 1:1000\u003cbr\u003e\nIF\/ICC: 1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: EP4 polyclonal antibody detects endogenous levels of EP4 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 65 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of EP4 expression in HEK293T (A), Hela (B), H1688 (C), mouse lung (D), mouse kidney (E) whole cell lysates., Immunofluorescent analysis of EP4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: PTGER4; EP4; EP4R\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human EP4.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989589987507,"sku":"BS66155-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989590020275,"sku":"BS66155-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3e963954-5319-4d62-b4e5-441bd096e041.jpg?v=1706095860","url":"https:\/\/afsbio.com\/products\/ep4-polyclonal-antibody-bs66155","provider":"AFSBio Inc.","version":"1.0","type":"link"}