{"product_id":"epcam-mouse-monoclonal-antibody-mb9010","title":"EPCAM Mouse Monoclonal Antibody -MB9010","description":"\u003cp\u003eEPCAM Mouse Monoclonal Antibody\u003c\/p\u003e \n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB9010-50, MB9010-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgM, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P16422\u003c\/p\u003e \n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IHC FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000-1:2000 ICC: 1:100-1:200\u003cbr\u003e\nIHC: 1:200-1:500 FC: 1:100-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The epithelial cell adhesion molecule Ep-CAM, which is also design atedtumor-associated calcium signal transducer 1 and MK-1, is a monomeric membrane glycoprotein that is expressed in most normal human epithelium and in most carcinomas. The human Ep-CAM gene encodes a 314 amino acid protein that is expressed as two forms, a 40 kDa major form and a 42 kDa minor form, which are reduced to 35 kDa upon treatment with the aminoglycosylation inhibitor tunicamycin. Ep-CAM is overexpressed in a variety of carcinomas and is, therefore, a potential target for the visualization and therapy of human solid tumours. Ep-CAM contains an extracellular domain containing two epidermal growth factor-like repeats, followed by a cysteinepoor region, which are necessary for the adhesion properties of the molecule.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProL affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of EPCAM and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 40 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of EPCAM Mouse mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: Hela whole cell lysate\u003cbr\u003e\n Lane 2: NIH\/3T3 whole cell lysate\u003cbr\u003e\n Lane 3: MCF7 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin., ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1\/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD326; EPCAM; GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: This antibody is produced by immunizing mice with recombinant protein corresponding to a region of EpCAM.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044036280499,"sku":"MB9010-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044036346035,"sku":"MB9010-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_4f71a859-8b82-4dc1-bfe6-4afe20005819.jpg?v=1707738838","url":"https:\/\/afsbio.com\/products\/epcam-mouse-monoclonal-antibody-mb9010","provider":"AFSBio Inc.","version":"1.0","type":"link"}