{"product_id":"ercc4-a822-polyclonal-antibody-bs2430","title":"ERCC4 (A822) Polyclonal Antibody-BS2430","description":"\u003cp\u003eERCC4 (A822) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2430-50, BS2430-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q92889\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Xeroderma Pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer, and it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF, which is also designated ERCC4 or ERCC11, is a 115 kDa protein that associates directly with the excision repair cross-complementing 1 (ERCC1) factor. ERCC-1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5’ incisions during DNA repair. The ERCC1-XPF endonuclease preferentially cleaves one strand of DNA between duplex and singlestranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: XPF (A822) polyclonal antibody detects endogenous levels of XPF protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of ERCC4 (A822) pAb at 1:500 dilution\u003cbr\u003e\nLane1:HEK293T whole cell lysate(40ug)\u003cbr\u003e\nLane2:MCF-7 whole cell lysate(40ug)\u003cbr\u003e\nLane3:U-87MG whole cell lysate(40ug)\u003cbr\u003e\nLane4:C6 whole cell lysate(40ug)\u003cbr\u003e\nLane5:CT26 whole cell lysate(40ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 117 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: ERCC4; ERCC11; XPF; DNA repair endonuclease XPF; DNA excision repair protein ERCC-4\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 796-840 of Human ERCC4.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910481744051,"sku":"BS2430-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910481776819,"sku":"BS2430-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ded9a9df-0a29-41ef-8095-f85c27ee112b.jpg?v=1703093342","url":"https:\/\/afsbio.com\/products\/ercc4-a822-polyclonal-antibody-bs2430","provider":"AFSBio Inc.","version":"1.0","type":"link"}