{"product_id":"erk-8-phospho-thr175-y177-rabbit-polyclonal-antibody-es5241","title":"ERK 8 (phospho Thr175\/Y177) Rabbit Polyclonal Antibody - ES5241","description":"\u003cp\u003eERK 8 (phospho Thr175\/Y177) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES5241-50, ES5241-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1.,function:In vitro, phosphorylates MBP.,PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.,PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.,similarity:Belongs to the protein kinase superfamily. CMGC Ser\/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with CSK\/c-Src, ABL1, RET and TGFB1I1.,tissue specificity:Widely expressed with a maximal expression in lung and kidney.,\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: MAPK15, ERK7, ERK8, Mitogen-activated protein kinase 15, MAP kinase 15, MAPK 15, Extracellular signal-regulated kinase 7, ERK-7, Extracellular signal-regulated kinase 8, ERK-8\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human ERK8 around the phosphorylation site of Thr175 and Tyr177. AA range:141-190\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: ERK8\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phospho-ERK 8 (T175\/Y177) Polyclonal Antibody detects endogenous levels of ERK 8 protein only when phosphorylated at T175\/Y177.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. ELISA: 1\/5000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e\n\n\u003cp\u003eFormlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Widely expressed with a maximal expression in lung and kidney,\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 60kD\u003c\/p\u003e\n\n\u003cp\u003eFields: IL-17 signaling pathway\u003c\/p\u003e\n\n\u003cp\u003eFunction: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein\u003cbr\u003e\nDomain: The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation\u003cbr\u003e\nDomain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases\u003cbr\u003e\nEnzyme Regulation: Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1\u003cbr\u003e\nFunction: In vitro, phosphorylates MBP\u003cbr\u003e\nPTM: Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro\u003cbr\u003e\nPTM: Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase\u003cbr\u003e\nSimilarity: Belongs to the protein kinase superfamily. CMGC Ser\/Thr protein kinase family. MAP kinase subfamily\u003cbr\u003e\nSimilarity: Contains 1 protein kinas\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cytoplasm, cytoskeleton, cilium basal body Cell junction, tight junction Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Cytoplasmic vesicle, autophagosome Golgi apparatus Nucleus Cytoplasm Cytoplasm, cytoskeleton, spindle Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936) Translocates to the nucleus upon activation (PubMed:20638370) At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (By similarity)\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: MAPK15\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Mitogen-activated protein kinase 15\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 332110\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: Q80Y86\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46313534619827,"sku":"ES5241-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46313534652595,"sku":"ES5241-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies_d75295e2-ffc1-4e0f-a3e7-87715c8983a9.jpg?v=1793190507","url":"https:\/\/afsbio.com\/products\/erk-8-phospho-thr175-y177-rabbit-polyclonal-antibody-es5241","provider":"AFSBio Inc.","version":"1.0","type":"link"}