{"product_id":"erk1-2-phospho-t202-t185-rabbit-monoclonal-antibody-mb66298","title":"ERK1\/2 (Phospho-T202\/T185) Rabbit Monoclonal Antibody - MB66298","description":"\u003cp\u003eERK1\/2 (Phospho-T202\/T185) Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66298-50, MB66298-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine\/threonine protein kinases involved in many cellμlar programs, such as cell proliferation, differentiation, motility, and death. The p44\/42 MAPK (Erk1\/2) signaling pathway can be activated in response to a diverse range of extracellμlar stimμli, including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimμlation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Mμltiple p44\/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2\/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202\/Tyr204 and Thr185\/Tyr187, respectively. Several downstream targets of p44\/42 have been identified, including p90RSK and the transcription factor Elk-1. p44\/42 are negatively regμlated by a family of dual-specificity (Thr\/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellμlar signal-regμlated kinase 1, ERK-1, Insμlin-stimμlated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubμle-associated protein 2 kinase, p44-ERK1, MAPK1, ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellμlar signal-regμlated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2\u003c\/p\u003e\n\n\u003cp\u003eClonality: Monoclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr202 of human ERK1\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB (1\/500 - 1\/1000), IP (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of ERK1\/2 (pT202\/T185) protein.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 44, 42 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: Tumor Angiogenesis, Regμlation of Apoptosis, ESC Pluripotency and Differentiation Signaling Pathway, Regμlation of Microtubμle Dynamics, ErbB HER Signaling, MAPK Erk in Growth and Differentiation Pathway, Inhibition of Apoptosis, Insμlin \u0026amp;Glucose Signaling, Mitochondrial Control of Apoptosis, T cell receptor signaling, TGFβ Smad signaling, MTOR signaling, Jak Stat, Warburg Effect, IL-6 Receptor Signaling, Translational Contral elF4 and p70 S6 Kinase\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: P27361, P28482\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946399097011,"sku":"MB66298-50","price":289.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946399129779,"sku":"MB66298-100","price":492.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/erk1-2-phospho-t202-t185-rabbit-monoclonal-antibody-mb66298","provider":"AFSBio Inc.","version":"1.0","type":"link"}