{"product_id":"erk7-monoclonal-antibody-mb67159","title":"ERK7 Monoclonal Antibody-MB67159","description":"\u003cp\u003eERK7 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB67159-50, MB67159-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q8TD08\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IF\/ICC (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking\/secretion and genome integrity, in a kinase activity-dependent manner.\u003cbr\u003e\nControls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation.\u003cbr\u003e\nRegulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling.\u003cbr\u003e\nPrevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins.\u003cbr\u003e\nUpon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion.\u003cbr\u003e\nBinds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA.\u003cbr\u003e\nRegulates DA transporter (DAT) activity and protein expression via activation of RhoA.\u003cbr\u003e\nIn response to H2O2 treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3'UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression.\u003cbr\u003e\nAlso functions in a kinase activity-independent manner as a negative regulator of growth (By similarity).\u003cbr\u003e\nPhosphorylates in vitro FOS and MBP.\u003cbr\u003e\nDuring oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (By similarity).\u003cbr\u003e\nInteracts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: This antibody is purified through a protein G column.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of ERK7 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 63 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of ERK7 expression in MCF7 (A) whole cell lysates., Immunofluorescent analysis of ERK7 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: ERK7; ERK8; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human ERK7. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989548667059,"sku":"MB67159-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989548699827,"sku":"MB67159-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ff1737f7-792c-446f-b892-4044965bef3c.jpg?v=1706095169","url":"https:\/\/afsbio.com\/products\/erk7-monoclonal-antibody-mb67159","provider":"AFSBio Inc.","version":"1.0","type":"link"}