{"product_id":"f-e3-free-estriol-elisa-kit-elk9646","title":"F-E3 (Free Estriol) ELISA Kit - ELK9646","description":"\u003cp\u003eF-E3 (Free Estriol) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK9646-48, ELK9646-96, ELK9646-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: F-E3; Free Estriol\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.225 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 40 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.63-40 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Endocrinology; Reproductive science; Hormone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with F-E3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F-E3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-E3 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212836503731,"sku":"ELK9646-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212836536499,"sku":"ELK9646-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200829612211,"sku":"ELK9646-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_51e50836-5498-44f1-9db0-2116f5b57138.png?v=1767065093","url":"https:\/\/afsbio.com\/products\/f-e3-free-estriol-elisa-kit-elk9646","provider":"AFSBio Inc.","version":"1.0","type":"link"}