FastGene Gel/PCR Extraction Kit Sample (4 preps)

FastGene Gel/PCR Extraction Kit Sample (4 preps)

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Details

FastGene® Gel/PCR Extraction Kits are designed for the extraction of DNA from agarose gels and for the purification of PCR products.

DNA fragments purified with FastGene® Gel/PCR Extraction Kits are ready for direct use in all common downstream applications, like sequencing, ligation and transformation, restriction digestion, microarray analysis, PCR and in vitro transcription.

Applications

The Figure demonstrates that the FastGene® Gel/PCR Extraction Kit shows up to 90% of DNA recovery.

FastGene Gel/PCR purification kit

Fig. 1: PCR fragments of 300 bp were purified from 40 µl of a PCR stock solution using FastGene® Gel/PCR Extraction Kit and a competitor kit, according to manufacturers protocol. DNA was eluted in 20 µl elution buffer and 5 µl of eluate was analyzed on a 1,5% TAE agarose

The DNA recovery from agarose gels using FastGene® Gel/PCR Extraction Kits is very high even for small fragments.

FastGene PCR purification

Fig. 2: 10 µl of a PCR stock solution with a 100 bp amplicon were applied on a TBE agarose gel. After electrophoresis the DNA was purified using a competitor kit and the FastGene® Gel/PCR Extraction Kit, according to manufacturers protocol. DNA was eluted in 20 µl elution buffer and 10 µl of eluate was analyzed on a 2% TAE agarose gel.
Lane 1: Unpurified PCR fragment; M = Marker
Lane 2,3 and 4: Supplier M
Lane 5,6 and 7: FastGene® Gel/PCR Extraction Kit

Protocol

Specifications

 Parameter Gel Extraction PCR Clean Up
Max Sample Vol 300mg Agarose Gel 100uL PCR Mix
Gel <2.5% TAE or TBE
Typical Recovery 70-80% 80-90%
Binding Capacity 10ug 10ug
DNA Fragment Length 50bp-10kb 50bp-10kb
Primer Removal <25bp
Elution Volume 20-50µL 20-50µL
Prep Time 20 minutes 20 minutes
Format Spin Column Spin Column

 

Downloads

Manual for FastGene Gel/PCR Extraction Kit

Application Note - Remove DNA of plant sample, derived cloning inhibitors from agarose gel

 Application NoteExtraction of large DNA fragments with the FastGene Gel/PCR Extraction Kit

Application Note - Purification of PCR products of fine roots (endospermatis) of Cherry Trees

Application Note - Insertion of moouse-derived osteoblast specific gene into expression vector

FAQs

Is the GP1 buffer of this kit available separately like for the Mini Kit Buffer Set?

A: We do not sell GP1 separately or as a Buffer Set.

What is the composition of the GP3 Buffer (does it contain EDTA or EtOH)?

The elution buffer GP3 contains just 10 mM Tris Buffer pH8 and no EtOH or EDTA.

Can I use water for the elution and what pH has the water to have?

You can use water but we would recommend to use the GP3 buffer. The pH should be between 7 and 8. If you buy water this should be OK. Please check the elution efficiency. If this is not OK check the pH of the water or use GP3.