FastGene® Gel/PCR Extraction Kits are designed for the extraction of DNA from agarose gels and for the purification of PCR products.
DNA fragments purified with FastGene® Gel/PCR Extraction Kits are ready for direct use in all common downstream applications, like sequencing, ligation and transformation, restriction digestion, microarray analysis, PCR and in vitro transcription.
The Figure demonstrates that the FastGene® Gel/PCR Extraction Kit shows up to 90% of DNA recovery.
Fig. 1: PCR fragments of 300 bp were purified from 40 µl of a PCR stock solution using FastGene® Gel/PCR Extraction Kit and a competitor kit, according to manufacturers protocol. DNA was eluted in 20 µl elution buffer and 5 µl of eluate was analyzed on a 1,5% TAE agarose
The DNA recovery from agarose gels using FastGene® Gel/PCR Extraction Kits is very high even for small fragments.
Fig. 2: 10 µl of a PCR stock solution with a 100 bp amplicon were applied on a TBE agarose gel. After electrophoresis the DNA was purified using a competitor kit and the FastGene® Gel/PCR Extraction Kit, according to manufacturers protocol. DNA was eluted in 20 µl elution buffer and 10 µl of eluate was analyzed on a 2% TAE agarose gel.
Lane 1: Unpurified PCR fragment; M = Marker
Lane 2,3 and 4: Supplier M
Lane 5,6 and 7: FastGene® Gel/PCR Extraction Kit
|Parameter||Gel Extraction||PCR Clean Up|
|Max Sample Vol||300mg Agarose Gel||100uL PCR Mix|
|Gel||<2.5% TAE or TBE|
|DNA Fragment Length||50bp-10kb||50bp-10kb|
|Prep Time||20 minutes||20 minutes|
|Format||Spin Column||Spin Column|
Application Note - Remove DNA of plant sample, derived cloning inhibitors from agarose gel
Application Note - Extraction of large DNA fragments with the FastGene Gel/PCR Extraction Kit
Application Note - Purification of PCR products of fine roots (endospermatis) of Cherry Trees
Application Note - Insertion of moouse-derived osteoblast specific gene into expression vector
Is the GP1 buffer of this kit available separately like for the Mini Kit Buffer Set?
A: We do not sell GP1 separately or as a Buffer Set.
What is the composition of the GP3 Buffer (does it contain EDTA or EtOH)?
The elution buffer GP3 contains just 10 mM Tris Buffer pH8 and no EtOH or EDTA.
Can I use water for the elution and what pH has the water to have?
You can use water but we would recommend to use the GP3 buffer. The pH should be between 7 and 8. If you buy water this should be OK. Please check the elution efficiency. If this is not OK check the pH of the water or use GP3.