Accurate Way for qPCR-based Quantification of Illumina® NGS-Libraries
For 500 Reactions
FastGene® NGS-Library Quantification Kit
The accurate quantification of next-generation sequencing (NGS) Illumina® Libraries is very important for maximizing data output and quality on every sequencing run. The FastGene® NGS-Library Quantification Kit contains all reagents for an accurate and reproducible qPCR-based quantification of Illumina® Libraries: The Kit comes with specific primers, qPCR Master Mix and DNA Standards.
- Quantification of Illumina® NGS-Libraries
- Preventing variability in the cluster density
Reproducible determination of sequenceable library
- Specific Primer included
Accurate and Sensitive qPCR-based Quantification
The FastGene® NGS Library Quantification kit was developed to reduce the variability in cluster density, resulting in improved sequencing results. The standards have been designed to allow a perfect concentration determination. The superior qPCR Master Mix included in the FastGene® NGS Library Quantification kit is able to detect even the smallest amount of libraries.
How did the NGS Library Quantification works?
The diluted library and the five known FastGene® NGS Standards are amplified using specific primers. The calibration curve is formed and used to determine the concentration of the sequencing competent libraries (Fig 1).
Fig. 1: Theoretical result of a library quantification (left) and calibration curve (right).
Specific detection of Libraries
FastGene® NGS Library Quantification kit comes with primers which are specific to the Illumina® adapters. Hence, only the libraries which have the adapter will be recognized by the FastGene® NGS Library Quantification kit.
FastGene® NGS Library Quantification kit comes with five very stable standards. These standards allow the formation of a standard curve to determine the concentration of a diluted library.
All components of the FastGene® NGS Library Quantification kit are submitted to repeated controls to ensure that there is no batch to batch difference. Additionally, freeze-thaw experiments of the components of the FastGene® NGS Library Quantification kit showed that no change was visible after repeatedly freeze-thawing the kit.
The FastGene® NGS-Library Quantification Kit contains:
- 5 mL 2x qPCR Ready Mix
- 5 Standards
- Illumina® specific Primers