FastGene® Plasmid Mini Kits are designed for rapid small scale isolation and purification of high copy and low copy plasmid DNA. The ready-to-use plasmid DNA is of high quality in low-salt Tris buffer and suitable for typical downstream applications: Cloning, sequencing, PCR, transformation and restriction analysis.
|Parameter||High-copy Plasmid||Low-copy Plasmid|
|Max. sample volume||1-5 ml ON-culture||5-10 ml ON-culture|
|Typical yield||< 25 µg||< 25 µg|
|Elution volume||50 µl||50 µl|
|Binding capacity||40 µg||40 µg|
|Size of vector||< 15 kb||< 15 kb|
|Prep time||26 min / 12 preps||26 min / 12 preps|
High yield – High purity – Fast preparation
The gel demonstrates that FastGene® Plasmid Mini Kits yield equal amount of plasmid DNA compared to other supplier with even more time consuming protocols. The preparation with the FastGene® Plasmid Mini Kit was performed by using the Fast Protocol.
Fig. 1: pBluescript plasmid DNA was isolated from a 1,4 ml E.coli culture (DH5α in LB medium) according to the recommended
procedures of different kits and eluted in 50 µl elution buffer. 2 µl of each eluate were loaded on a 0,7% TAE agarose gel.
Fig.2: Typical fluorescent capillary sequencing result from pBluescript plasmid DNA purified using the FastGene® Plasmid Mini Kit. Cycle sequencing reaction was performed on an ABI3100 sequenzer. 1 µl of purified plasmid DNA was used as a template. Dye terminators were removed by FastGene® Dye Terminator Removal Kit
Application Note - Purification of plasmids using FastGene® Plasmid Mini Kit
Application Note - Sample preparation for in situ hybridization of targeted tissue specific genes in medeka fish