FastGene® Taq ReadyMix (2X) is a ready-to-use cocktail containing all components for PCR, except primers and template. The 2X ReadyMix contains FastGene® Taq DNA Polymerase (1 U per 50 μl reaction), FastGene® Taq Buffer (1X), dNTPs (0.2 mM of each dNTP at 1X), MgCl2 (1.5 mM at 1X) and stabilizers.
FastGene® Taq ReadyMix comes with two inert tracking dyes to enable direct loading of PCR products onto agarose gels for analysis by electrophoresis, without the need to add a DNA loading solution.
FastGene® Taq ReadyMix with dye is recommended when reaction products are analyzed by agarose gel electrophoresis:
Fig. 4: FastGene® Taq reactions with 1X Loading Dye Reaction Buffer A.Volumes above wells indicate the volume of the PCR reaction loaded on the gel. B.On a 1% agarose gel, the blue dye migrates at the same rate as a 5 kb DNA fragment, and the yellow dye migrates at 75 bp.FastGene® Taq ReadyMix PCR Kits are shipped on ice packs, depending on the country of destination. Upon arrival, store kit components at -20 °C in a constant temperature freezer. FastGene®Taq ReadyMixes contain iso-stabilizers and may not freeze solidly, even when stored at -20 °C. This will not affect the shelf-life of the product.