{"product_id":"fbxw7-polyclonal-antibody-bs90499","title":"FBXW7 Polyclonal Antibody-BS90499","description":"\u003cp\u003eFBXW7 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90499-50, BS90499-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q969H0(Human) Q8VBV4(Mouse) D3Z902(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB\t1:500 - 1:2000  IHC\t1:50 - 1:200  IF\t1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites\/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein affinity purified.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: FBXW7 polyclonal antibody detects endogenous levels of FBXW7 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 70 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of FBXW7 on different lysates using anti-FBXW7 antibody at 1\/1,000 dilution.\u003cbr\u003e\n Positive control:\u003cbr\u003e\n Lane 1: Mouse spleen tissue \u003cbr\u003e\n Lane 2: Rat lung tissue\u003cbr\u003e\n Lane 3: HepG2, ICC staining FBXW7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: hAgo, FBXW7, FBW7, AGO, Archipelago, Archipelago homolog, Archipelago, Drosophila, homolog of, CDC4, CDC4, S.cerevisiae, homolog of, DKFZp686F23254, F box and WD 40 domain protein 7 (archipelago homolog, Drosophila), F box and WD 40 domain protein 7, F box and WD repeat domain containing 7, F box protein FBW7, F box protein FBX30, F box protein SEL 10, F box protein SEL10, F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, F-box\/WD repeat-containing protein 7, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, FBXW7, FLJ16457, hAgo, hCdc4, Homolog of C elegans sel 10, Homolog of C.elegans sel10, SEL 10, SEL-10, SEL10,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979811950771,"sku":"BS90499-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979811983539,"sku":"BS90499-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_af83e870-09ac-440a-bac5-d9ffb0685dc6.jpg?v=1705753177","url":"https:\/\/afsbio.com\/products\/fbxw7-polyclonal-antibody-bs90499","provider":"AFSBio Inc.","version":"1.0","type":"link"}