{"product_id":"fen1-7h8-monoclonal-antibody-mb0179","title":"FEN1 (7H8) Monoclonal Antibody-MB0179","description":"\u003cp\u003eFEN1 (7H8) Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB0179-50, MB0179-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P39748\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eFEN1 (7H8) Monoclonal Antibody-MB0179\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000\u003cbr\u003e\nICC: 1:1000~500\u003c\/p\u003e\n\n\u003cp\u003eBackground: DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. FEN-1 (for flap endonuclease) is an endonuclease that specifically cleaves the 5' flap structure of DNA in the process of DNA repair. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of FEN1 and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Predicted band size:42KDa \u003cbr\u003e\nObserved band size:42KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blotDetection of FEN-1 in Hela,Jurkat,3T3,COS7,PC-12,C6,Raw264.7,Raji and CHO-K1 cellLysates using FEN-1 mouse mAb (1:1000 diluted) ., Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400) .\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Flap endonuclease 1; FEN-1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1; FEN1; RAD2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Purified recombinant human FEN-1 protein fragments expressed in E.coli.\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42962828427443,"sku":"MB0179-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42962828460211,"sku":"MB0179-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_425622c4-b4c9-4cbc-9d28-b718705cedb2.jpg?v=1705319191","url":"https:\/\/afsbio.com\/products\/fen1-7h8-monoclonal-antibody-mb0179","provider":"AFSBio Inc.","version":"1.0","type":"link"}