{"product_id":"fibrillarin-polyclonal-antibody-bs90511","title":"Fibrillarin Polyclonal Antibody-BS90511","description":"\u003cp\u003eFibrillarin Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90511-50, BS90511-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P22087(Human) P35550(Mouse)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:1,000\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Fibrillarin is a widely occurring, basic, nonhistone protein that is localized exclusively in the fibrillar region of the nucleolus, including both the dense fibrillar and the fibrillar center regions. Fibrillarin is also expressed in HeLa cells, 3T3 cells, and human peripheral blood lymphocytes. In metaphase and anaphase, fibrillarin is found on putative chromosomal nucleolar regions (NORs). During telophase, fibrillarin is an early marker for the site of the newly forming nucleolus. The structure of fibrillarin includes an RNA-binding domain and an RNP consensus sequence, which is consistent with the association of fibrillarin with the U3 small nucleolar RNA. Fibrillarin is involved in processing rRNA transcripts in the nucleolus.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Peptide affinity purified.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Fibrillarin polyclonal antibody detects endogenous levels of Fibrillarin protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 34 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Fibrillarin on different cell lysates using anti-Fibrillarin antibody at 1\/1,000 dilution.\u003cbr\u003e\n Positive control:\u003cbr\u003e\n Lane 1: Hela \u003cbr\u003e\n Lane 2: HepG2\u003cbr\u003e\n Lane 3: 293T \u003cbr\u003e\n Lane 4: NIH-3T3, Flow cytometric analysis of HL-60 cells with Fibrillarin antibody at 1\/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: FBL, FIB1, FLRN, 34 kD nucleolar scleroderma antigen, 34 kDa nucleolar scleroderma antigen, Fbl, FBRL, FIB, FIB1, FLRN, Histone-glutamine methyltransferase, Nop1p, RNA U3 small nucleolar interacting protein 1, RNU3IP1, rRNA 2' O methyltransferase fibrillarin, rRNA 2'-O-methyltransferase fibrillarin,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Synthetic peptide of C-terminal human Fibrillarin.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979812540595,"sku":"BS90511-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979812573363,"sku":"BS90511-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_f7b0738f-17ee-4654-b1e1-eeb0d7380e4a.jpg?v=1705753195","url":"https:\/\/afsbio.com\/products\/fibrillarin-polyclonal-antibody-bs90511","provider":"AFSBio Inc.","version":"1.0","type":"link"}