{"product_id":"flag-tag-polyclonal-antibody-bt-ap15612","title":"FLAG-tag Polyclonal Antibody - BT-AP15612","description":"\u003cp\u003eFLAG-tag Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μL, 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BT-AP15612-20, BT-AP15612-50, BT-AP15612-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.\u003c\/p\u003e \n\n\u003cp\u003eResearch Areas: Tags and cell markers\u003c\/p\u003e\n\n\u003cp\u003eTarget Protein: Flag\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ELISA\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Species independent\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eFull Product Name: Flag-Tag DDDDK TAG, DDDDK-TAG\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C for 1 year\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml\u003c\/p\u003e\n\n\u003cp\u003eSynonyms: Flag-Tag DDDDK TAG, DDDDK-TAG; DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"BT Lab","offers":[{"title":"20μL","offer_id":45238871064755,"sku":"BT-AP15612-20","price":151.2,"currency_code":"CAD","in_stock":true},{"title":"50μL","offer_id":45238871097523,"sku":"BT-AP15612-50","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45238871130291,"sku":"BT-AP15612-100","price":385.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/logo_3159648a-28c0-49de-88c4-aa5c33634cbf.png?v=1769386028","url":"https:\/\/afsbio.com\/products\/flag-tag-polyclonal-antibody-bt-ap15612","provider":"AFSBio Inc.","version":"1.0","type":"link"}