{"product_id":"flag-tag-polyclonal-antibody-ra20821","title":"FLAG-tag Polyclonal Antibody - RA20821","description":"\u003cp\u003eFLAG-tag Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RA20821-50, RA20821-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.\u003c\/p\u003e \n\n\u003cp\u003eCondition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eStorage Instructions: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eRecommended dilutions: Western Blot: 1\/1000 - 1\/3000. ELISA: 1\/20000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB; ELISA\u003c\/p\u003e\n\n\u003cp\u003eSpecies Cross-Reactivity: Species independent\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Reed Bio","offers":[{"title":"50μL","offer_id":45119075483827,"sku":"RA20821-50","price":336.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45119075516595,"sku":"RA20821-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/logo_49ed3992-598d-4855-a8c5-944c6040f7e3.png?v=1773350751","url":"https:\/\/afsbio.com\/products\/flag-tag-polyclonal-antibody-ra20821","provider":"AFSBio Inc.","version":"1.0","type":"link"}