{"product_id":"foxo1a-acetyl-lys245-rabbit-pab-es20066","title":"FoxO1A (Acetyl Lys245) rabbit pAb - ES20066","description":"\u003cp\u003eFoxO1A (Acetyl Lys245) rabbit pAb\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES20066-50, ES20066-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: disease:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2; 13) (q35; q14) with PAX3; translocation t (1; 13) (p36; q14) with PAX7. The resulting protein is a transcriptional activator., function:Transcription factor., PTM:Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Contains 1 fork-head DNA-binding domain., subcellular location:Shuttles between cytoplasm and nucleus., subunit:Interacts with LRPPRC., tissue specificity:Ubiquitous.,\u003c\/p\u003e \n\n\u003cp\u003eAlternate Name: Forkhead box protein O1 (Forkhead box protein O1A; Forkhead in rhabdomyosarcoma)\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB; ELISA\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB 1:1000-2000 ELISA 1:5000-20000\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human; Mouse; Rat\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthesized peptide derived from human FoxO1A (Acetyl Lys245)\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 72kD\u003c\/p\u003e\n\n\u003cp\u003eHuman Gene ID: 2308\u003c\/p\u003e\n\n\u003cp\u003eHuman SWISS Prot NO: Q12778\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cytoplasm. Nucleus. Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB\/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"50μL","offer_id":45202426822835,"sku":"ES20066-50","price":207.2,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45202426855603,"sku":"ES20066-100","price":347.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_5681f495-4196-4a64-a069-6a61079ec95f.png?v=1767128963","url":"https:\/\/afsbio.com\/products\/foxo1a-acetyl-lys245-rabbit-pab-es20066","provider":"AFSBio Inc.","version":"1.0","type":"link"}