{"product_id":"foxo4-phospho-s262-polyclonal-antibody-bs67196","title":"FOXO4 (phospho-S262) Polyclonal Antibody -BS67196","description":"\u003cp\u003eFOXO4 (phospho-S262) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS67196-50, BS67196-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P98177\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eFOXO4 (phospho-S262) Polyclonal Antibody -BS67196\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IHC (1\/50 - 1\/200)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 157% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of FOXO4 with a phosphorylation site at S262 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 70 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of FOXO4 (pS262) expression in mouse muscle (A), rat brain (B), rat muscle (C) whole cellLysates., Immunohistochemical analysis of FOXO4 (pS262) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXO4 with a phosphorylation site at S262. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957293846707,"sku":"BS67196-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957293879475,"sku":"BS67196-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_19d327b8-5a08-44bc-a030-856976996504.jpg?v=1705144479","url":"https:\/\/afsbio.com\/products\/foxo4-phospho-s262-polyclonal-antibody-bs67196","provider":"AFSBio Inc.","version":"1.0","type":"link"}