{"product_id":"frizzled-4-polyclonal-antibody-bs60963","title":"Frizzled-4 Polyclonal Antibody-BS60963","description":"\u003cp\u003eFrizzled-4 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS60963-50, BS60963-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Frizzled-4; Fz-4; hFz4; FzE4; CD344; FZD4\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9ULV1\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Frizzled-4 is a 537 amino acid protein encoded by the human gene FZD4. Frizzled-4 acts as a receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Frizzled-4 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and\/or in differentiated tissues. Frizzled-4 also plays a critical role in retinal angiogenesis. Frizzled-4 is virtually ubiquitously expressed with greatest amounts found in adult heart, skeletal muscle, ovary, and fetal kidney.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Frizzled-4 polyclonal antibody detects endogenous levels of Frizzled-4 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 60 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Frizzled-4 pAb at 1:500 dilution\u003cbr\u003e\nLane 1: A549 whole cell lysate(40ug)\u003cbr\u003e\nLane 2: Hela whole cell lysate(40ug)\u003cbr\u003e\nLane 3: CT26 whole cell lysate(40ug)\u003cbr\u003e\nLane 4: PC12 whole cell lysate(40ug)\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide corresponding to residues in Human Frizzled-4.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42913804124339,"sku":"BS60963-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42913804157107,"sku":"BS60963-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_307b812a-f0bb-4569-804d-039944bff4b7.jpg?v=1703267061","url":"https:\/\/afsbio.com\/products\/frizzled-4-polyclonal-antibody-bs60963","provider":"AFSBio Inc.","version":"1.0","type":"link"}