{"product_id":"frizzled-7-y87-polyclonal-antibody-bs2774","title":"Frizzled-7 (Y87) Polyclonal Antibody-BS2774","description":"\u003cp\u003eFrizzled-7 (Y87) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2774-50, BS2774-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Frizzled7; Frizzled 7; Fz-7; hFz7; FzE3; FZD7\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O75084\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nIF: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The mammalian homolog of frizzled, as well as several secreted mammalian frizzled-related proteins: FRP-1 (also designated SARP2), FRP-2 (also designated SARP1), FRP-3, FRP-4 and SARP3 (also designated FRP-5), have been identified. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser\/Thr-xxx-Val motif. They function as receptors for Wnt. frizzled-7 has a Lys-Thr-X-X-X-Trp motif which is involved in the activation of the Wnt\/beta-catenin signaling pathway. frizzled-7 is an integral membrane protein showing a high expression in adult skeletal muscle, fetal kidney, fetal lung, adult heart, brain and placenta. It is specifically expressed in squamous cell esophageal carcinomas.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Frizzled-7 (Y87) polyclonal antibody detects endogenous levels of Frizzled-7 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 64, 85 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Frizzled-7 (Y87) pAb at 1:500 dilution\u003cbr\u003e\nLane 1: PC3 whole cell lysate(20ug)\u003cbr\u003e\nLane 2: HEK293T whole cell lysate(40ug)\u003cbr\u003e\nLane 3: 3T3-L1 whole cell lysate(40ug)\u003cbr\u003e\nLane 4: PC12 whole cell lysate(40ug)\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 61-110 of Human Frizzled-7.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42911798296755,"sku":"BS2774-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42911798329523,"sku":"BS2774-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3869010d-b8b0-4520-8d41-6d03ef9991c2.jpg?v=1703179403","url":"https:\/\/afsbio.com\/products\/frizzled-7-y87-polyclonal-antibody-bs2774","provider":"AFSBio Inc.","version":"1.0","type":"link"}