{"product_id":"frizzled-8-polyclonal-antibody-bs2700","title":"Frizzled-8 Polyclonal Antibody-BS2700","description":"\u003cp\u003eFrizzled-8 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2700-50, BS2700-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9H461\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:1000~1:2000\u003c\/p\u003e \n\n\u003cp\u003eBackground: Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and\/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Frizzled-8 polyclonal antibody detects endogenous levels of Frizzled-8 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 73 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Frizzled-8 (FZD8) at 1:1000 dilution\u003cbr\u003e\n Lane 1: HEK293T whole cell lysate (30ug)\u003cbr\u003e\n Lane 2: A549 whole cell lysate (30ug)\u003cbr\u003e\n Lane 3: The Stomach tissue lysate of Mouse (30ug)\u003cbr\u003e\n Lane 4: The Spleen tissue lysate of Rat (30ug)\u003cbr\u003e\n Lane 5: BV2 whole cell lysate (30ug)\u003cbr\u003e\n Lane 6: PC12 whole cell lysate (30ug)\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Fz-8; hFz8; FZD8; FZ-8; hFZ8; Frizzled 8; Frizzled8\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human Frizzled-8.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42987091656883,"sku":"BS2700-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42987091689651,"sku":"BS2700-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3b6dd018-ebcb-402e-b934-26b0f435132f.jpg?v=1706008973","url":"https:\/\/afsbio.com\/products\/frizzled-8-polyclonal-antibody-bs2700","provider":"AFSBio Inc.","version":"1.0","type":"link"}