{"product_id":"fubp3-monoclonal-antibody-mb67176","title":"FUBP3 Monoclonal Antibody-MB67176","description":"FUBP3 Monoclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: MB67176-50, MB67176-100\n\nProduct: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.\n\nSwiss-Prot: Q96I24\n\nHost: Mouse\n\nReactivity: Human\n\nApplications: WB, IF\/ICC\n\nAll Applications: WB (1\/500 - 1\/1000), IF\/ICC (1\/10 - 1\/50)\n\nBackground: Activation of FUSE, the far-upstream element, is required for the proper expression of the mammalian gene c-Myc in undifferentiated cells. The binding of FBP (FUSE-binding protein or far upstream element binding protein) to FUSE is necessary for c-Myc expression, indicating that FBP functions as a growth-dependent regulator of c-Myc expression. Isolated from proliferating HL60 cells, FBP, FBP2, and FBP3 comprise a family of single-stranded DNAbinding proteins that specifically bind to FUSE elements. The FBP transcription factors share a conserved central DNA-binding domain and show significant homology in their carboxyl-terminal activation domains. Expression of FBP is detected in undifferentiated cells and is substantially decreased following cellular differentiation.\n\nPurification and Purity: This antibody is purified through a protein G column.\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Recognizes endogenous levels of FUBP3 protein.\n\nBioworld Molecular Weight: ~ 62 kDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of FUBP3 expression in Hela (A), U251 (B) whole cell lysates., Immunofluorescent analysis of FUBP3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).\n\nAlternative Name: FBP3; Far upstream element-binding protein 3; FUSE-binding protein 3\n\nImmunogen: Recombinant fusion protein of human FUBP3. The exact sequence is proprietary.\n\nConjugate: Unconjugated\n\nModification: Unmodified","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989548765363,"sku":"MB67176-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989548798131,"sku":"MB67176-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3ff2cc06-dc14-4c87-84cb-9fba584e8170.jpg?v=1706095171","url":"https:\/\/afsbio.com\/products\/fubp3-monoclonal-antibody-mb67176","provider":"AFSBio Inc.","version":"1.0","type":"link"}