{"product_id":"fzr1-polyclonal-antibody-bs7791","title":"FZR1 Polyclonal Antibody-BS7791","description":"\u003cp\u003eFZR1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50 µl, 100 µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS7791-50, BS7791-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9UM11\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eFZR1 Polyclonal Antibody-BS7791\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000  |  IF\/ICC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Substrate-specific adapter for the anaphase promoting complex\/cyclosome (APC\/C E3 ubiquitin-protein ligase complex. Associates with the APC\/C in late mitosis, in replacement of CDC20, and activates the APC\/C during anaphase and telophase. The APC\/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1\/S transition FZR1 is phosphorylated, leading to its dissociation from the APC\/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC\/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC\/C to target the DNA-end resection factor RBBP8\/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8\/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ over error-free, RBBP8-mediated homologous recombination (HR.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 55KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of 293T cells, using FZR1 antibody at 1:500 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Enhanced Kit.\u003cbr\u003e\nExposure time: 180s., Immunofluorescence analysis of HeLa cells using FZR1 antibody. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: FZR1; CDC20C; CDH1; FZR; FZR2; HCDH; HCDH1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human FZR1(NP_057347.2).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42968466686131,"sku":"BS7791-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42968466718899,"sku":"BS7791-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_8b54bbce-51c7-41d9-b86d-18045f33e9e5.jpg?v=1705428198","url":"https:\/\/afsbio.com\/products\/fzr1-polyclonal-antibody-bs7791","provider":"AFSBio Inc.","version":"1.0","type":"link"}