{"product_id":"gap43-polyclonal-antibody-bs67791","title":"GAP43 Polyclonal Antibody-BS67791","description":"\u003cp\u003eGAP43 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS67791-50, BS67791-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P17677\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IF\/ICC (1\/50 - 1\/200)\u003c\/p\u003e\n\n\u003cp\u003eBackground: GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity. Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca2+ and affects the ability of GAP43 to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex. Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's disease.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of GAP43 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 43 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of GAP43 expression in U251MG (A), mouse heart (B), rat brain (C) whole cell lysates., Immunofluorescent analysis of GAP43 staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein corresponding to human GAP43. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42987094999219,"sku":"BS67791-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42987095031987,"sku":"BS67791-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_7a36f838-b077-42ea-968b-f6f4c68a24c5.jpg?v=1706009083","url":"https:\/\/afsbio.com\/products\/gap43-polyclonal-antibody-bs67791","provider":"AFSBio Inc.","version":"1.0","type":"link"}