{"product_id":"gap43-polyclonal-antibody-bs76974","title":"GAP43 Polyclonal Antibody-BS76974","description":"GAP43 Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS76974-50, BS76974-100\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSwiss-Prot: P17677\n\nHost: Rabbit\n\nReactivity: Mouse, Rat\n\nApplications: WB, IHC\n\nAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:200\n\nBackground: The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Unmodification \n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of extracts of various cell lines, using GAP43 antibody at 1:1000 dilution.\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\n Lysates\/proteins: 25ug per Lane.\n Blocking buffer: 3% non-fat dry milk in TBST.\n Detection: ECL Basic Kit.\n Exposure time: 90s., Immunohistochemistry of paraffin-embedded mouse brain using GAP43 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat brain using GAP43 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.\n\nAlternative Name: B-50; PP46; GAP43\n\nImmunogen: Recombinant fusion protein of human GAP43(NP_002036.1).\n\nConjugate: Unconjugated\n\nModification: Unmodified ","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42993874075827,"sku":"BS76974-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42993874108595,"sku":"BS76974-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ec2406e1-6b7c-48ca-8b78-bce76480b126.jpg?v=1706184573","url":"https:\/\/afsbio.com\/products\/gap43-polyclonal-antibody-bs76974","provider":"AFSBio Inc.","version":"1.0","type":"link"}