{"product_id":"gapdh-monoclonal-antibody-mb9231","title":"GAPDH Monoclonal Antibody-MB9231","description":"\u003cp\u003eGAPDH Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB9231-50, MB9231-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgM, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P04406\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003cbr\u003e\nApplications: WB, ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:5,000-1:10,000\u003cbr\u003e\nICC:1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProL affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: GAPDH monoclonal antibody detects endogenous levels of GAPDH protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 36 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1\/5000 dilution.\u003cbr\u003e\n Positive control: \u003cbr\u003e\n Lane 1: HepG2 \u003cbr\u003e\n Lane 2: Hela \u003cbr\u003e\n Lane 3: PC12 \u003cbr\u003e\n Lane 4: NIH\/3T3 \u003cbr\u003e\n Lane 5: MCF-7 \u003cbr\u003e\n Lane 6: Rabbit liver \u003cbr\u003e\n Lane 7: Zebrafish\u003cbr\u003e\n , ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: GAPDH, G3P, 38 kDa BFA-dependent ADP-ribosylation substrate, aging associated gene 9 protein, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, Epididymis secretory sperm binding protein Li 162eP, G3P, G3PD, G3PDH, GAPD, GAPDH, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL-S-162eP, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38 component, OCT1 coactivator in S phase, 38-KD component, peptidyl cysteine S nitrosylase GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, wu:fb33a10,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: peptide\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42975432409267,"sku":"MB9231-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42975432442035,"sku":"MB9231-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_0c768e79-6a02-476f-af4c-590b2b259b7c.jpg?v=1705665971","url":"https:\/\/afsbio.com\/products\/gapdh-monoclonal-antibody-mb9231","provider":"AFSBio Inc.","version":"1.0","type":"link"}