{"product_id":"gcs-α-1-d423-polyclonal-antibody-bs3670","title":"GCS-α-1 (D423) Polyclonal Antibody-BS3670","description":"\u003cp\u003eGCS-α-1 (D423) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS3670-50, BS3670-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q02108\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e \n\n\u003cp\u003eBackground: Guanylate cyclases belong to the adenylyl cyclase class-4\/guanylyl cyclase family. There are two forms of guanylate cyclase, a soluble form (GCS or sGC), which act as receptors for nitric oxide and a membrane-bound receptor form (GC), which are peptide hormone receptors. The GC-C protein is composed of an extracellular domain, a single transmembrane domain, and a cytoplasmic region consisting of a kinase-like domain and a catalytic domain. It is expressed as two differentially glycosylated forms, a 130 kDa precursor form present in the endoplasmic reticulum and a 145 kDa form present on the plasma membrane. Ligand binding to the extracellular domain of GC-C promotes the accumulation of cGMP. GC-C acts as the receptor for heatstable enterotoxins, small peptides secreted by some pathogenic strains of E. coli that cause severe secretory diarrhea. GC-C also binds to guanylin and uroguanylin peptides, which modulate renal function in response to oral salt load.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: GCS-α-1 (D423) polyclonal antibody detects endogenous levels of GCS-α-1 protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of GCS-α-1 (D423) pAb at 1:500 dilution\u003cbr\u003e\nLane 1: PC3 whole cell lysate (20ug)\u003cbr\u003e\nLane 2: A549 whole cell lysate (20ug)\u003cbr\u003e\nLane 3: HEK293T whole cell lysate (20ug)\u003cbr\u003e\nLane 4: PMVEC whole cell lysate (40ug)\u003cbr\u003e\nLane 5: The Brain tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of GCS-α-1 (D423) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 77 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Guanylate cyclase soluble subunit alpha-3; GCS-alpha-3; EC=4.6.1.2; GCS-alpha-1; Soluble guanylate cyclase large subunit; GUC1A3; GUCSA3; GUCY1A1; GUCY1A3; GCSα1; GCS-α1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human GCS-α-1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953238741171,"sku":"BS3670-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953238773939,"sku":"BS3670-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_9e9985c7-3f2b-4268-967f-2a75478b14c7.jpg?v=1705057426","url":"https:\/\/afsbio.com\/products\/gcs-%ce%b1-1-d423-polyclonal-antibody-bs3670","provider":"AFSBio Inc.","version":"1.0","type":"link"}