{"product_id":"gcs-β-1-v21-polyclonal-antibody-bs1158","title":"GCS-β-1 (V21) Polyclonal Antibody-BS1158","description":"\u003cp\u003eGCS-β-1 (V21) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1158-50, BS1158-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q02153\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IP,\u003c\/p\u003e  \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nIP: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Guanylate cyclases belong to the adenylyl cyclase class-4\/guanylyl cyclase family. There are two forms of guanylate cyclase, a soluble form (GCS or sGC), which act as receptors for nitric oxide and a membrane-bound receptor form (GC), which are peptide hormone receptors. The GC-C protein is composed of an extracellular domain, a single transmembrane domain, and a cytoplasmic region consisting of a kinase-like domain and a catalytic domain. It is expressed as two differentially glycosylated forms, a 130 kDa precursor form present in the endoplasmic reticulum and a 145 kDa form present on the plasma membrane. Ligand binding to the extracellular domain of GC-C promotes the accumulation of cGMP. GC-C acts as the receptor for heatstable enterotoxins, small peptides secreted by some pathogenic strains of E. coli that cause severe secretory diarrhea. GC-C also binds to guanylin and uroguanylin peptides, which modulate renal function in response to oral salt load.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: GCS-β-1 (V21) polyclonal antibody detects endogenous levels of Guanylate Cyclase β1\/3 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 70 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of GCS-β-1 (V21) polyclonal antibody at 1:500 dilution\u003cbr\u003e\n Lane 1: THP-1 whole cell lysate\u003cbr\u003e\n Lane 2: sp2\/0 whole cell lysate\u003cbr\u003e\n Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of GCS-β-1 (V21) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of the Brain tissue lysate of Mouse using GCS-β-1 (V21) pAb (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using GCS-β-1 (V21) pAb #BS1158. “↑”(supernatant); “↓”(deposition)\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Guanylate cyclase soluble subunit beta-1; GCS-beta-1; Guanylate cyclase soluble subunit beta-3; GCS-beta-3; Soluble guanylate cyclase small subunit; GUCY1B3; GUC1B3; GUCSB3; GUCY1B1; GCS-β1; GCS β1; GCSβ1; GCS-β3; GCS β3; GCSβ3; GCS-β-3\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to the N-terminus of Human GCS-β-1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42993789305011,"sku":"BS1158-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42993789337779,"sku":"BS1158-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e64347a5-04b1-4a95-a29f-7d9fe0fcf7c9.jpg?v=1706183587","url":"https:\/\/afsbio.com\/products\/gcs-%ce%b2-1-v21-polyclonal-antibody-bs1158","provider":"AFSBio Inc.","version":"1.0","type":"link"}