{"product_id":"gctm-2-monoclonal-antibody-mb66709","title":"GCTM-2 Monoclonal Antibody-MB66709","description":"\u003cp\u003eGCTM-2 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66709-50, MB66709-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O00592\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/1000 - 1\/5000), IF\/ICC (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eBackground: TRA-1-60 and TRA-1-81 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells. TRA-1-60(S) reacts with a neuraminidase sensitive epitope of a proteoglycan, while TRA-1-81 reacts with a neuraminidase insensitive epitope on the same antigen. Recently this antigen has been proposed to be a form of the protein podocalyxin. TRA-1-60 is also detected in the serum of patients with germ cell tumors.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of GCTM-2 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 57 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of GCTM-2 expression in A549 (A) whole cell lysates., Immunofluorescent analysis of GCTM-2 staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: PCLP; PCLP1; Podocalyxin; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GCTM-2. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42987090378931,"sku":"MB66709-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42987090411699,"sku":"MB66709-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_cda9a158-8a46-4fb3-8121-b6fb855e6f81.jpg?v=1706008932","url":"https:\/\/afsbio.com\/products\/gctm-2-monoclonal-antibody-mb66709","provider":"AFSBio Inc.","version":"1.0","type":"link"}