{"product_id":"gelsolin-polyclonal-antibody-bs76980","title":"Gelsolin Polyclonal Antibody-BS76980","description":"Gelsolin Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS76980-50, BS76980-100\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSwiss-Prot: P06396\n\nHost: Rabbit\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, IHC, IF\/ICC\n\nAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:200   |   IF\/ICC,1:50 - 1:200\n\nBackground: The protein encoded by this gene binds to the \"plus\" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Unmodification \n\nBioworld Molecular Weight: 86KDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of extracts of various cell lines, using Gelsolin antibody at 1:1000 dilution.\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\n Lysates\/proteins: 25ug per Lane.\n Blocking buffer: 3% non-fat dry milk in TBST.\n Detection: ECL Basic Kit.\n Exposure time: 30s., Immunohistochemistry of paraffin-embedded human colon carcinoma using Gelsolin Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human liver cancer using Gelsolin Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse spleen using Gelsolin Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.\n\nAlternative Name: GSN; ADF; AGEL; gelsolin\n\nImmunogen: Recombinant fusion protein of human Gelsolin(NP_000168.1).\n\nConjugate: Unconjugated\n\nModification: Unmodified ","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43047642890419,"sku":"BS76980-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43047642923187,"sku":"BS76980-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_8a34bf79-9854-4df1-b287-261ca6cf7522.jpg?v=1707826641","url":"https:\/\/afsbio.com\/products\/gelsolin-polyclonal-antibody-bs76980","provider":"AFSBio Inc.","version":"1.0","type":"link"}