{"product_id":"gip-polyclonal-antibody-bs7977","title":"GIP Polyclonal Antibody-BS7977","description":"\u003cp\u003eGIP Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS7977-50, BS7977-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P09681\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eGIP Polyclonal Antibody-BS7977\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ELISA IHC, IF ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500-1:2000\u003cbr\u003e\nELISA: 1:5000-1:10000\u003cbr\u003e\nIHC: 1:100-1:500\u003cbr\u003e\nIF: 1:100-1:500\u003cbr\u003e\nICC: 1:100-1:500\u003c\/p\u003e\n\n\u003cp\u003eBackground: Glucose-dependent insulinotropic polypeptide (GIP) is a major physiologic factor in the augmentation of the insulin response to oral glucose. GIP is a peptide hormone that is released postprandially from the small intestine and acts in concert with glucagon-like peptide (GLP)-1 to potentiate glucose-induced insulin secretion from the pancreatic β-cell. GIP has been shown to increase adenylyl cyclase activity, elevate intracellular calcium levels, and stimulate a mitogen-activated protein kinase pathway in the pancreatic β-cell. Additionally, nutrient protein provides a potent stimulus for GIP expression, an effect that occurs at the posttranslational level and may be mediated in part through the acid-stimulatory properties of protein. GIP release is demonstrated predominantly after ingestion of carbohydrate and fat and the effects of acid on GIP are consistent with a role for GIP as an enterogastrone.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: GIP polyclonal antibody detects endogenous levels of GIP protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 17 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GIP) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C., Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (GIP) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Gastric Inhibitory polypeptide; Gastric inhibitory polypeptide precursor; GIP; Glucose dependent insulinotropic polypeptide; Gastric Inhibitory Peptide; GIP_HUMAN; Glucose-dependent insulinotropic polypeptide; Incretin hormone.\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: KLH conjugated synthetic peptide derived from human GIP:52-93\/153.\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42962721734835,"sku":"BS7977-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42962721800371,"sku":"BS7977-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ffa25bda-3ce3-4bbe-9ee6-67643c50c883.jpg?v=1705318557","url":"https:\/\/afsbio.com\/products\/gip-polyclonal-antibody-bs7977","provider":"AFSBio Inc.","version":"1.0","type":"link"}