{"product_id":"git1-polyclonal-antibody-bs90574","title":"GIT1 Polyclonal Antibody-BS90574","description":"\u003cp\u003eGIT1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90574-50, BS90574-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9Y2X7(Human) Q68FF6(Mouse) Q9Z272(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:1,000\u003cbr\u003e\nIHC:1:50-1:200\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade. GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, which are involved in vesicular trafficking. GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, thus reducing b2-adrenergic receptor signaling.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: GIT1 polyclonal antibody detects endogenous levels of GIT1 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 85 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of GIT1 on A549 cell using anti-GIT1 antibody at 1\/500 dilution., Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GIT1 antibody. Counter stained with hematoxylin.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: ARF GAP GIT1, CAT-1, CAT1, GIT1, ARF GAP GIT1, ARF GTPase activating protein GIT1, ARF GTPase-activating protein GIT1, CAT 1, CAT-1, CaT1, Cool associated and tyrosine phosphorylated protein 1, Cool-associated and tyrosine-phosphorylated protein 1, G protein coupled receptor kinase interacting ArfGAP 1, G protein coupled receptor kinase interactor 1, G protein-coupled receptor kinase-interactor 1, GIT1, GIT1, GRK interacting protein 1, GRK-interacting protein 1,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979815981235,"sku":"BS90574-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979816014003,"sku":"BS90574-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_741d196d-50fc-4aca-bacb-83c3d5a36c1d.jpg?v=1705753313","url":"https:\/\/afsbio.com\/products\/git1-polyclonal-antibody-bs90574","provider":"AFSBio Inc.","version":"1.0","type":"link"}