{"product_id":"gli1-polyclonal-antibody-bs90575","title":"Gli1 Polyclonal Antibody-BS90575","description":"\u003cp\u003eGli1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90575-50, BS90575-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P08151(Human)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:1,000\u003cbr\u003e\nIHC:1:50-1:200\u003cbr\u003e\nICC:1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. GLI-1 (GLI family zinc finger 1), also known as Glioma-associated oncogene or oncogene GLI, is a 1,106 amino acid protein that localizes to both the cytoplasm and nucleus, and belongs to the GLI C2H2-type zinc-finger protein family. GLI-1 acts as a transcriptional activator and is thought to play a role in craniofacial development. GLI-1 exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 12q13.3.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Gli1 polyclonal antibody detects endogenous levels of Gli1 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 150 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Gli1 on SH-SY-5Y cells lysates using anti-Gli1 antibody at 1\/1,000 dilution., ICC staining Gli1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: GLI1, Gli 1, GLI, GLI family zinc finger 1, GLI Kruppel family member 1, gli1, GLI1, Glioma associated oncogene 1, Glioma associated oncogene homolog 1 (zinc finger protein), Glioma associated oncogene homolog, Glioma-associated oncogene, Oncogene GLI, Zfp 5, Zfp5, Zinc finger protein GLI 1, Zinc finger protein GLI1,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979816046771,"sku":"BS90575-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979816079539,"sku":"BS90575-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_1211b29a-2c7e-42d6-9521-ebed65e53ac3.jpg?v=1705753316","url":"https:\/\/afsbio.com\/products\/gli1-polyclonal-antibody-bs90575","provider":"AFSBio Inc.","version":"1.0","type":"link"}