{"product_id":"glucosidase-iiβ-k117-polyclonal-antibody-bs2646","title":"Glucosidase IIβ (K117) Polyclonal Antibody-BS2646","description":"\u003cp\u003eGlucosidase IIβ (K117) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2646-50, BS2646-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Glucosidase 2 subunit beta; 80K-H protein; Glucosidase II subunit beta; Protein kinase C substrate 60.1 kDa protein heavy chain; PKCSH; PRKCSH; G19P1\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P14314\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nIF: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ). The α and β subunits form a defined heterodimeric complex with a molecular weight about 161 kDa. Glucosidase IIα is the catalyitc core of the enzyme and can function independently of the β subunit. The sequence of Glucosidase IIβ encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. The phosphorylated form of Glucosidase IIβ is localized in the plasma membrane and is highly expressed in FGF stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIβ was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIβ retains the complete complex in the ER.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Glucosidase IIβ (K117) polyclonal antibody detects endogenous levels of Glucosidase IIβ protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 59, 80 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Glucosidase IIβ (K117) pAb at 1:500 dilution\u003cbr\u003e\nLane 1: The Testis tissue lysate of Mouse(40ug)\u003cbr\u003e\nLane 2: The Testis tissue lysate of Rat(40ug)\u003cbr\u003e\nLane 3: SGC7901 whole cell lysate(40ug)\u003cbr\u003e\nLane 4: HCT116 whole cell lysate(40ug)\u003cbr\u003e\nLane 5: A549 whole cell lysate(40ug)\u003cbr\u003e\nLane 6: MCF-7 whole cell lysate(40ug)\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 90-130 of Human Glucosidase IIβ.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42911797313715,"sku":"BS2646-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42911797346483,"sku":"BS2646-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ecb9aa1c-830f-46c2-973a-a67586eca16c.jpg?v=1703179373","url":"https:\/\/afsbio.com\/products\/glucosidase-ii%ce%b2-k117-polyclonal-antibody-bs2646","provider":"AFSBio Inc.","version":"1.0","type":"link"}