{"product_id":"glyrs-monoclonal-antibody-mb66982","title":"GlyRS Monoclonal Antibody-MB66982","description":"\u003cp\u003eGlyRS Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66982-50, MB66982-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P41250\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/1000 - 1\/2000), IF\/ICC (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) .Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis .\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: This antibody is purified through a protein G column.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of GlyRS protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 85 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of GlyRS expression in HT1080 (A), Jurkat (B), Ramos (C) whole cell lysates., Immunofluorescent analysis of GlyRS staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Glycine--tRNA ligase; Diadenosine tetraphosphate synthetase; AP-4-A synthetase; Glycyl-tRNA synthetase; GlyRS\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human GlyRS. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989548241075,"sku":"MB66982-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989548273843,"sku":"MB66982-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_779eac96-0f66-4b46-b1e4-8addbfd977d9.jpg?v=1706095157","url":"https:\/\/afsbio.com\/products\/glyrs-monoclonal-antibody-mb66982","provider":"AFSBio Inc.","version":"1.0","type":"link"}