{"product_id":"gp91-phox-nox2-polyclonal-antibody-bs90591","title":"gp91-phox\/NOX2 Polyclonal Antibody-BS90591","description":"\u003cp\u003egp91-phox\/NOX2 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90591-50, BS90591-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P04839(Human) Q61093(Mouse)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IHC FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:2,000\u003cbr\u003e\nICC:1:50-1:200\u003cbr\u003e\nIHC:1:500-1:1,000\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet they have distinct expression patterns. gp91-phox is expressed in eosinophils, neutro-phils, monocytes and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, and low expression is also detected in the uterus and prostate. Mox1 is also upregulated in vascular smooth-muscle cells in response to PDGF stimulation, which collectively indicates that Mox1 may function analogously to gp91-phox, yet regulate the NADPH superoxide production in non-phagocytic cells.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Peptide affinity purified.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: gp91-phox\/NOX2 polyclonal antibody detects endogenous levels of gp91-phox\/NOX2 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 65 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of NOX2\/gp91phox on A549 cell lysates using anti-NOX2\/gp91phox antibody at 1\/500 dilution., ICC staining NOX2\/gp91phox in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Cytochrome b558 subunit beta, CYBB, NOX2, AMCBX2, CGD, CGD91-phox, CY24B, CYBB, Cytochrome b 245, beta polypeptide, Cytochrome b(558) beta chain, Cytochrome b(558) subunit beta, Cytochrome b-245 heavy chain, Cytochrome b558 subunit beta, GP91 PHOX, gp91-1, gp91-phox, GP91PHOX, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, NOX2, p22 phagocyte B-cytochrome, P91 PHOX, p91-PHOX, Superoxide-generating NADPH oxidase heavy chain subunit,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Synthetic peptide corresponding to C-terminal human NOX2\/gp91phox.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979817029811,"sku":"BS90591-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979817062579,"sku":"BS90591-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_8bb3f41e-11ee-4384-9371-5a343704541e.jpg?v=1705753353","url":"https:\/\/afsbio.com\/products\/gp91-phox-nox2-polyclonal-antibody-bs90591","provider":"AFSBio Inc.","version":"1.0","type":"link"}