{"product_id":"gp91-phox-nox2-polyclonal-antibody-bs90592","title":"gp91-phox\/NOX2 Polyclonal Antibody-BS90592","description":"\u003cp\u003egp91-phox\/NOX2 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90592-50, BS90592-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P04839(Human) Q61093(Mouse) EntrezGene:66021(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000-1:2,000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane, where they associate with the flavocytochrome cytochrome b558 to form the active enzyme complex. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet they have distinct expression patterns. gp91-phox is expressed in eosinophils, neutro-phils, monocytes and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, and low expression is also detected in the uterus and prostate. Mox1 is also upregulated in vascular smooth-muscle cells in response to PDGF stimulation, which collectively indicates that Mox1 may function analogously to gp91-phox, yet regulate the NADPH superoxide production in non-phagocytic cells.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of NOX2 on differentLysates using anti-NOX2 antibody at 1\/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: THP-1 Lane 3: HepG2\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 65 kDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: gp91-phox\/NOX2 polyclonal antibody detects endogenous levels of gp91-phox\/NOX2 protein.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Cytochrome b558 subunit beta, CYBB, AMCBX2, CGD, CGD91-phox, CY24B, CYBB, Cytochrome b 245, beta polypeptide, Cytochrome b(558) beta chain, Cytochrome b(558) subunit beta, Cytochrome b-245 heavy chain, Cytochrome b558 subunit beta, GP91 PHOX, gp91-1, gp91-phox, GP91PHOX, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, NOX2, p22 phagocyte B-cytochrome, P91 PHOX, p91-PHOX, Superoxide-generating NADPH oxidase heavy chain subunit,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42939200110771,"sku":"BS90592-50","price":415.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42939200143539,"sku":"BS90592-100","price":640.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_c747c17e-3bbf-4232-b03c-b8d98f7d9fef.jpg?v=1704545009","url":"https:\/\/afsbio.com\/products\/gp91-phox-nox2-polyclonal-antibody-bs90592","provider":"AFSBio Inc.","version":"1.0","type":"link"}