{"product_id":"granuphilin-polyclonal-antibody-bs62493","title":"Granuphilin Polyclonal Antibody-BS62493","description":"\u003cp\u003eGranuphilin Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS62493-50, BS62493-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q96C24\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500~1:1000 IHC:1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Granuphilin, also designated synaptotagmin-like protein 4 or exophilin 2, is a member of the synaptotagmin-like protein family. In rodents, two isoforms have been characterized as granuphlin-a and granuphilin-b, and alternative splicing in human has also been described. Granuphilin-b is a shorter isoform which contains a different C-terminal sequence than the longer isoform. Granuphilin is part of a ternary complex consisting of Syntaxin 1A (STX1A) and Rab 27A. The interaction between granuphilin and Syntaxin 1A on the plasma membrane is regulated by Rab 27A. Granuphilin is a peripheral membrane protein mainly expressed in pancreatic β-cells and in the pituitary gland. It has been detected on secretory granules and close to the plasma membrane. In the pancreas, granuphilin interacts with Insulin-containing vesicles while in both the pancreas and pituitary, granuphilin modulates the secretion of hormones and the exocytosis of dense-core granules. Overexpression of granuphilin enhances basal Insulin secretion but also inhibits high K+-induced Insulin secretion. The effect of granuphilin on Insulin secretion may be impaired by a mutation that disrupts the binding to either Rab 27A or Syntaxin 1A, making granuphilin a possible regulator in the exocytotic pathway\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Granuphilin polyclonal antibody detects endogenous levels of Granuphilin protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Granuphilin polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLaneA:CT26 whole cell lysate\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 76 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Synaptotagmin-like protein 4; Exophilin-2; Granuphilin; SYTL4\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human Granuphilin.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910447501491,"sku":"BS62493-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910447534259,"sku":"BS62493-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_70dff0e8-f992-4492-80c3-7559d475cab9.jpg?v=1703092573","url":"https:\/\/afsbio.com\/products\/granuphilin-polyclonal-antibody-bs62493","provider":"AFSBio Inc.","version":"1.0","type":"link"}