{"product_id":"grid2-polyclonal-antibody-bs77893","title":"GRID2 Polyclonal Antibody-BS77893","description":"GRID2 Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS77893-50, BS77893-100\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSwiss-Prot: O43424\n\nHost: Rabbit\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, IF\/ICC\n\nAll Applications: WB,1:500 - 1:2000   |   IF\/ICC,1:50 - 1:200\n\nBackground: The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans. [provided by RefSeq, Apr 2014]\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Unmodification \n\nBioworld Molecular Weight: 113KDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of extracts of Mouse brian, using GRID2 antibody at 1:1000 dilution.\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\n Lysates\/proteins: 25ug per Lane.\n Blocking buffer: 3% non-fat dry milk in TBST.\n Detection: ECL Basic Kit.\n Exposure time: 10s., Immunofluorescence analysis of C6 cells using GRID2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of L929 cells using GRID2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U-2 OS cells using GRID2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.\n\nAlternative Name: GluD2; SCAR18; GRID2\n\nImmunogen: A synthetic peptide of human GRID2(NP_001501.2).\n\nConjugate: Unconjugated\n\nModification: Unmodified ","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43047653441715,"sku":"BS77893-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43047653474483,"sku":"BS77893-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3b456704-9aab-4a70-bdd8-289bc8939c73.jpg?v=1707826894","url":"https:\/\/afsbio.com\/products\/grid2-polyclonal-antibody-bs77893","provider":"AFSBio Inc.","version":"1.0","type":"link"}