{"product_id":"grp1-k400-polyclonal-antibody-bs2942","title":"GRP1 (K400) Polyclonal Antibody-BS2942","description":"\u003cp\u003eGRP1 (K400) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2942-50, BS2942-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Cytohesin-3; ARF nucleotide-binding site opener 3; Protein ARNO3; General receptor of phosphoinositides 1; Grp1; PH; SEC7 and coiled-coil domain-containing protein 3; ARNO3; GRP1; PSCD3; CYTH3\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O43739\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: GRP1 (general receptor for phosphoinositides-1) contains a Pleckstrin homology (PH) domain as well as a Sec7 domain. The PH domain has high binding affinity for phosphatidylinositol 3,4,5-trisphosphate Ptdlns(3,4,5)P3), while the Sec7 homology domain is responsible for catalyzing guanine nucleotide exchange of ADP-ribosylation factor (ARF) proteins. GRP1 co-localizes with ARF6 and catalyzes GTP\/GDP exchange on ARF6. It is known to interact with Ptdlns(3,4,5)P3 localized to the plasma membrane in vitro and may also be a Ptdlns(3,4,5)P3 receptor. Additionally, GRP1 may regulate protein sorting and membrane trafficking through interaction with the guanosine triphosphate ARF, and may control cell adhesion through interaction with integrins.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: GRP1 (K400) polyclonal antibody detects endogenous levels of GRP1 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 48 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of GRP1 (K400) pAb at 1:2000 dilution\u003cbr\u003e\nLane 1: 3T3-L1 whole cell lysate(40ug)\u003cbr\u003e\nLane 2: H9C2 whole cell lysate(40ug)\u003cbr\u003e\nLane 3: A2780 whole cell lysate(40ug)\u003cbr\u003e\nLane 4: H1792 whole cell lysate(40ug)\u003cbr\u003e\nLane 5: A375 whole cell lysate(20ug)\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 371-420 of Human GRP1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42911799410867,"sku":"BS2942-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42911799443635,"sku":"BS2942-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_f6221bf6-498d-4ebc-80ec-2486f624b074.jpg?v=1703179435","url":"https:\/\/afsbio.com\/products\/grp1-k400-polyclonal-antibody-bs2942","provider":"AFSBio Inc.","version":"1.0","type":"link"}