{"product_id":"grp75-s664-polyclonal-antibody-bs1153","title":"GRP75 (S664) Polyclonal Antibody-BS1153","description":"\u003cp\u003eGRP75 (S664) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1153-50, BS1153-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P38646\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC\/IF: 1:50~1:200\u003cbr\u003e\nIP: 1:10~1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery. They are involved in chaperoning nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: GRP75 (S664) polyclonal antibody detects endogenous levels of GRP75 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 75 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of GRP75 (S664) pAb at 1:5000 dilution\u003cbr\u003e\n Lane 1: A549 whole cell lysate (20ug)\u003cbr\u003e\n Lane 2: HepG2K562 whole cell lysate (20ug)\u003cbr\u003e\n Lane 3: The Brain tissue lysate of Mouse (40ug)\u003cbr\u003e\n Lane 4: The Brain tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of GRP75 (S664) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of A549 whole cell lysate using GRP75 (S664) pAb (Sepharose Bead Conjugate) #BD0047(Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4 and Lane 5). Lane 1 is 20% input. The western blot was probed using GRP75 (S664) pAb #BS1153. “↑”（supernatant); “↓”（deposition）\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Stress-70 protein, mitochondrial; 75 kDa glucose-regulated protein; GRP-75; Heat shock 70 kDa protein 9; Mortalin; MOT; Peptide-binding protein 74; PBP74; HSPA9; GRP75; HSPA9B; mt-HSP70; HSP A9\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids C-terminus of Human GRP75.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42993789042867,"sku":"BS1153-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42993789075635,"sku":"BS1153-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_4291f335-805d-400a-96c4-1b85bd20b4d7.jpg?v=1706183582","url":"https:\/\/afsbio.com\/products\/grp75-s664-polyclonal-antibody-bs1153","provider":"AFSBio Inc.","version":"1.0","type":"link"}