{"product_id":"grp78-p641-polyclonal-antibody-bs1154","title":"GRP78 (P641) Polyclonal Antibody-BS1154","description":"\u003cp\u003eGRP78 (P641) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1154-50, BS1154-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P11021\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC\/IF: 1:50~1:200\u003cbr\u003e\nIP: 1:10~1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: GRP78 (P641) polyclonal antibody detects endogenous levels of GRP78 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 78 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of GRP78 (P641) polyclonal antibody at 1:500 dilution\u003cbr\u003e\n Lane 1: L02 whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: HCT116 whole cell lysate (40ug)Lane 3: HCT116 whole cell lysate (40ug)\u003cbr\u003e\n Lane 3: AML-12 whole cell lysate (40ug)\u003cbr\u003e\n Lane 4: PC12 whole cell lysate (40ug)Lane 3: HCT116 whole cell lysate (40ug)\u003cbr\u003e\n Lane 5: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of GRP78 (P641) pAbb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of L02 whole cell lysate using GRP78 (P641) pAb (Sepharose Bead Conjugate) #BD0047(Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4). Lane 1 is 20% input. The western blot was probed using GRP78 (P641) pAb #BS11542. “↑”（supernatant); “↓”（deposition）\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; HSPA5; GRP78\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 611-660 of Human GRP78.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42993789141171,"sku":"BS1154-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42993789173939,"sku":"BS1154-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_cc7105f3-f5e9-497e-9335-a7468c5582be.jpg?v=1706183585","url":"https:\/\/afsbio.com\/products\/grp78-p641-polyclonal-antibody-bs1154","provider":"AFSBio Inc.","version":"1.0","type":"link"}