{"product_id":"gtf2h1-polyclonal-antibody-bs71475","title":"GTF2H1 Polyclonal Antibody-BS71475","description":"\u003cp\u003eGTF2H1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50 µl, 100 µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS71475-50, BS71475-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P32780\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:1000   |   IF\/ICC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Component of the general transcription and DNA repair factor IIH (TFIIH core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 65kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using GTF2H1 antibody at 1:1000 dilution. \u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. \u003cbr\u003e\nLysates\/proteins: 25ug per lane. \u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST. \u003cbr\u003e\nDetection: ECL Basic Kit. \u003cbr\u003e\nExposure time: 1s., Immunofluorescence analysis of U-2 OS cells using GTF2H1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: GTF2H1; BTF2; P62; TFB1; TFIIH\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human GTF2H1(NP_001135779.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42968518525107,"sku":"BS71475-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42968518557875,"sku":"BS71475-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_f3899fcc-bb3a-44f0-9331-1f1e07f98cdc.jpg?v=1705430142","url":"https:\/\/afsbio.com\/products\/gtf2h1-polyclonal-antibody-bs71475","provider":"AFSBio Inc.","version":"1.0","type":"link"}