{"product_id":"ha-tag-polyclonal-antibody-ap0005","title":"HA-tag Polyclonal Antibody-AP0005","description":"\u003cp\u003eHA-tag Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: AP0005-50, AP0005-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: All\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:2000~1:10000\u003cbr\u003e\nIF: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: HA-tag polyclonal antibody detects over-expressed or recombinant proteins containing the HA epitope tag.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: N\/A\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of HA-tag polyclonal antibody at 1:5000 dillution\u003cbr\u003e\n Lane 1: HEK293T whole cell lysate, untransfected (40μg)\u003cbr\u003e\n Lane 2: HEK293T whole cell lysate, transfected with pRK5-(HA)2-RhoA(35μg), IF image of AP0005 stained HEK293T cells, transfected with pcDNA3.1-p62 #PPL00549-2b. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody HA-tag pAb #AP0005(1:200) at 5µg\/ml overnight at +4°C. The secondary antibody (Green) wasGoat anti-Rabbit IgG (H+L) -FITC#BS10950 used at a 1\/1000 dilution for 1h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: HA epitope tag; HA Tag; hemagglutinin\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide HA-tag.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989583237299,"sku":"AP0005-50","price":263.54,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989583270067,"sku":"AP0005-100","price":424.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_192149f2-39a9-400d-96fc-effc78bbddb0.jpg?v=1706095745","url":"https:\/\/afsbio.com\/products\/ha-tag-polyclonal-antibody-ap0005","provider":"AFSBio Inc.","version":"1.0","type":"link"}