{"product_id":"hamster-tnf-a-tumor-necrosis-factor-alpha-elisa-kit-elk0928","title":"Hamster TNF-a (Tumor Necrosis Factor-alpha) ELISA Kit - ELK0928","description":"\u003cp\u003eHamster TNF-a (Tumor Necrosis Factor-alpha) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0928-48, ELK0928-96, ELK0928-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.09 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 200 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.13-200 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cytokine; Tumor immunity; Infection immunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster TNF-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster TNF-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster TNF-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster TNF-a in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211549343923,"sku":"ELK0928-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211549376691,"sku":"ELK0928-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200646209715,"sku":"ELK0928-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_4afaaf19-162a-4d04-9d58-754654dd91eb.png?v=1767060884","url":"https:\/\/afsbio.com\/products\/hamster-tnf-a-tumor-necrosis-factor-alpha-elisa-kit-elk0928","provider":"AFSBio Inc.","version":"1.0","type":"link"}