{"product_id":"hck-phospho-tyr410-rabbit-polyclonal-antibody-es5721","title":"Hck (phospho Tyr410) Rabbit Polyclonal Antibody - ES5721","description":"\u003cp\u003eHck (phospho Tyr410) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES5721-50, ES5721-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010],\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: IHC, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: HCK, Tyrosine-protein kinase HCK, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK\/p60-HCK, p59Hck, p61Hck\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human HCK around the phosphorylation site of Tyr410. AA range:381-430\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: Hck\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phospho-Hck (Y410) Polyclonal Antibody detects endogenous levels of Hck protein only when phosphorylated at Y410.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Immunohistochemistry: 1\/100 - 1\/300. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/20000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e\n\n\u003cp\u003eFormlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Detected in monocytes and neutrophils (at protein level), Expressed predominantly in cells of the myeloid and B-lymphoid lineages, Highly expressed in granulocytes, Detected in tonsil,\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight (Da): 60kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Kaposi sarcoma-associated herpesvirus infection\u003c\/p\u003e\n\n\u003cp\u003eFunction: Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate\u003cbr\u003e\nDomain: The SH3 domain mediates binding to HIV-1 Nef\u003cbr\u003e\nFunction: May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils\u003cbr\u003e\nPTM: Isoform p59-HCK contains a N-myristoyl glycine at position 3 (By similarity). Isoform p59-HCK contains a S-palmitoyl cysteine at position 3\u003cbr\u003e\nSimilarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily\u003cbr\u003e\nSimilarity: Contains 1 protein kinase domain\u003cbr\u003e\nSimilarity: Contains 1 SH2 domain\u003cbr\u003e\nSimilarity: Contains 1 SH3 domain\u003cbr\u003e\nSubunit: May interact (via SH3 domain) with HIV-1 Nef and Vif. This interaction would stimulates its tyrosine-kinase activity. Interacts (via SH3 domain) with HEV ORF3 protein\u003cbr\u003e\ntissu\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: [Isoform 1]: Lysosome Membrane, Lipid-anchor Cell projection, podosome membrane, Lipid-anchor Cytoplasm, cytosol Associated with specialized secretory lysosomes called azurophil granules At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated, [Isoform 2]: Cell membrane, Lipid-anchor Membrane, caveola, Lipid-anchor Cell junction, focal adhesion Cytoplasm, cytoskeleton Golgi apparatus Cytoplasmic vesicle Lysosome Nucleus 20% of this isoform is associated with caveolae Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3 Colocalizes with the actin cytoskeleton at focal adhesions, Cytoplasmic vesicle, secretory vesicle Cytoplasm, cytosol\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: HCK\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Tyrosine-protein kinase HCK\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 15162\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: P08103\u003c\/p\u003e\n\n\u003cp\u003eRat Gene ID: 25734\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: P50545\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46313562636467,"sku":"ES5721-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46313562669235,"sku":"ES5721-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies_d75295e2-ffc1-4e0f-a3e7-87715c8983a9.jpg?v=1793190507","url":"https:\/\/afsbio.com\/products\/hck-phospho-tyr410-rabbit-polyclonal-antibody-es5721","provider":"AFSBio Inc.","version":"1.0","type":"link"}